将增强型绿色荧光蛋白(EGFP)的cDNA插入了小鼠白化C57BL/6胚胎干细胞系中基因的第二个外显子,紧接在原起始密码子之后。同时,一个含loxP标记的诺卡因抗性基因座被插入在EGFP序列之后。通过与C57BL/6背景的EIIa-Cre小鼠杂交,这个带有诺卡因抗性元件的靶向载体被剔除了。(来源:J:156990)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
插入
--
1
1
9

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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