germ line(生殖细胞)中,通过Cre介导的重组被用来去除exon 3。通过在杂合子小鼠胚胎成纤维细胞提取物中进行 Western blot 分析,确认了这个等位基因没有蛋白质表达。(来源:J:137482)
查看原文 参与反馈
Germ line, cre-mediated recombination was used to remove exon 3. Lack of protein expression from this allele was confirmed by western blot analysis on heterozygous mouse embryonic fibroblast extracts. (J:137482)