Bcl2l11^tm3Rjd

A floxed neo cassette was inserted within intron 4, and a specific mutation in exon 4 was introduced by homologous recombination. Mutation resulted in one site of JNK phosphorylation (Thr-112) in exon 4 to be substituted with an Ala (T112A). The neo cassette was then excised with cre recombines leaving a single loxP site in intron 4. Expression of normal amounts of mutant proteins was confirmed by immunoblot. (J:137061)