germ line(生殖细胞)、通过Cre介导的重组被用来去除neo cassette以及4到11号外显子。蛋白产物的缺失通过在肝提取物中进行 Western blot 分析得到了确认(来源:J:137101)
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Germ line, cre-mediated recombination was used to remove the neo cassette and exons 4 through 11. The absence of protein product was confirmed by western blot analysis on liver extracts. (J:137101)