通过在5'端插入LoxP位点,3'端包含Frt-PGK-neomycin-Frt-LoxP表达座,构建了一个靶向载体。随后,这些带有内切位点的转基因小鼠与CAGG-Flpe转基因小鼠杂交,以产生可遗传的突变型(Floxed allele)。来源:(J:137185)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count