在exon 11的700bp位置插入了一个floxed neo cassette,并在exon 10上游额外插入了一个loxP位点。通过将这种特定的基因型(A floxed neo cassette in exon 11 with an upstream loxP site)用pMC-cre表达载体转染定向的ES细胞,产生了Gria1tm1Rsp(J:55695)。
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A floxed neo cassette was inserted 700 bp into exon 11 along with an additional loxP site inserted upstream of exon 10. This allele was used to generate Gria1tm1Rsp by transfecting targeted ES cells with a cre-expressing vector (pMC-cre). (J:55695)