将一个新的 neo cassette 插入到了 exons 3 中,导致了框架移位并引入了一个早发的终止密码子。蛋白质产物的缺失通过在原纤维细胞提取物中进行 Western blot 分析得到了确认。(来源:J:136890)
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A neo cassette was inserted into exon 3 leading to a frame shift and the introduction of a premature stop codon. The absence of protein product was confirmed by western blot analysis on primary fibroblast extracts. (J:136890)