Nrxn1^tm2Sud

A loxP site was inserted into the 5' end of a modified exon 18, which contains an EGFP reporter gene followed by FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette and left the modified exon 18 floxed. The GFP insert does not affect the expression of neurexin 1 gene, but it is very difficult to detect GFP signals in the tissue by immunohistochemistry or direct fluorescence. (J:224592)