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An frt-flanked betageo cassette with a 3' loxP site and a 5' SV40-polyA tail was inserted downstream of exon 8 and an additional loxP site was inserted upstream of exon 8. Transient flp expression in ES cells was used to remove the betageo cassette and leave exon 8 floxed. Cre expression in germline cells removed exon 8 and created a null allele. No gene product was detected by PCR in homozygous null embryos. (J:134633)