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The Mir106b, Mir93, and Mir25 genes were replaced by an Frt-flanked neomycin cassette. No homozygous mutants are obtained if the neomycin is left in place, but Flp-mediated deletion of neo produces phenotypically normal homozygous mice. Since the Mirn106b-25 cluster resides in the 13th intron of the Mcm7 gene, these results suggest that the neomycin cassette interferes with expression of Mcm7. (J:134861)