Tg(Itgax-TGFBR2)1Flv

The human TGFBRIII (Tgfbr2 transforming growth factor, beta receptor II) gene sequence between nucleotides -7 to +573 encoding the extracellular and transmembrane regions of TGFBR2 (dominant negative, dnTGFBR2) was inserted into exon 3 of the rabbit beta globin gene in the plasmid Itgax promoter vector pDOI-5. The construct was injected into (C57BL/6 x C3H)F1 hybrid fertilized eggs. RT-PCR confirmed expression of dnTGFBR2 in Itgax (CD11c)-expressing cells including subsets of natural killer cell (NK) and dendritic cell (DC) subsets. No expression is detected in NKT cells, T cells or B cells. Expression is sufficient to block TFG beta signaling through the functional receptor exclusively in DCs and NK cells. (J:98953)