Rela^tm1.1Gho

By homologous recombination in ES cells, endogenous DNA sequence for the serine 276 residue in exon 7 of Rela gene was replaced with codon for alanine followed by a neo cassette flanked by two loxP sites inserted into intron after Exon 7. A neo cassette was then deleted either in the recombined ES cells by transfection of cre-recombinase or in mice by crossing with splicer mice harboring a Cre transgene, leaving only single loxP sites. The presence of the mutation was confirmed by Northern blot, RT-PCR and DNA sequencing. (J:134684)