在E11.5胚胎的提取物中,通过 Western blot 分析确认了外显子1和2被替换了新插入的neo cassette,没有蛋白质产物产生。(来源:J:135610)
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Exons 1 and 2 were replaced with a neo cassette. The absence of protein product was confirmed by western blot analysis on E11.5 embryo extracts. (J:135610)