部分exon2和exon3被替换成带有IRES-lacZ和floxed neo元件。通过小鼠的生殖细胞,通过cre介导的重组来去除neo cassette。蛋白产物的缺失通过 western blot 分析确认。(来源:J:135686)
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Part of exon 2 and exon 3 were replaced with an IRES-lacZ and floxed neo cassette. Germ line, cre-mediated recombination was used to remove the neo cassette. The absence of protein product was confirmed by western blot analysis. (J:135686)