在信号序列下游,插入了一个loxP-IRES Tak PGK-Neo cassette,通过 Western blot 在脑组织提取物中确认了没有蛋白质产物产生。(来源:J:135421)
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A loxP-IRES Tak PGK-Neo cassette was inserted into the open reading frame downstream of the signal sequence. The absence of protein product was confirmed by western blot analysis on brain extracts. (J:135421)