Del(4Cdkn2a-Cdkn2b)1Brn

Cre-mediated recombination in ES cells with a loxP site and a stop codon inserted in exon 1 alpha of Cdkn2a and with exons 1 and 2 of Cdkn2b replaced with a PGK-hyg cassette as well as a 5' loxP site resulted in a deletion encompassing the promoter of Cdkn2b and the 5' half of the Cdkn2a exon 1a. The absence of p15Ink4b, p16Ink4a and p19Arf protein products was confirmed by western blot analysis on mouse embryonic fibroblasts. (J:125189)