Rspo1^tm1.1Ktom

This allele was made using sequential targeting events. The first targeting event resulted in the insertion of a floxed neo cassette into exon 2 and the second event resulted in the replacement of exons 5 through 6 with a floxed puromycin resistance cassette. Transient cre expression was used to remove the selection cassettes and the remaining coding region of exon 2 and exon 3. The final allele lacks the entire coding region. The absence of transcript was confirmed by RT-PCR on ovary and testis extracts. (J:133934)