Gt(ROSA)26Sor^{tm4(CAG-lacZ,-EGFP)Dym}

A dual recombinase-responsive indicator cassette was knocked into the gene locus. This cassette consisted of (upstream to downstream): a cytomegalovirus enhancer/chicken beta-actin promoter; a splice acceptor; a loxP-flanked PGK-neomycin resistance cassette followed by four consecutive polyadenylation sequences; a FRT-flanked nuclear lacZ encoding gene followed by five consecutive polyadenylation sequences; and a bicistronic element encoding for wheat germ agglutinin (WGA) and a farnesylated, membrane-localized form of eGFP. This allele expresses nlacZ after cre-mediated excision of the neo cassette and turns off nlacZ and turns on WGA and eGFP expression after both cre- and Flp-recombinases are expressed. (J:133039)