在exon 1的上游插入了一个带有loxP切割位点的诺姆选择性 cassette,然后在exon的下游额外插入了另一个loxP位点。通过瞬时表达Cre重组酶,去除了诺姆选择性 cassette,保留了loxP位点修饰的exon 1。靶向正确性通过Southern blotting方法进行了确认。(来源:J:132855)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S/SvEv
Targeted
插入
--
1
--
29

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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