在exon 2的上游插入了loxP位点,而在exon 3下游则插入了loxP标记的诺卡因选择子 cassette。通过瞬时表达Cre重组酶,去除了诺卡因 cassette,使得exons 2和3被loxP位点包裹。靶向正确性通过Southern blot检测确认。(来源:J:132855)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S/SvEv
Targeted
插入
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1
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28

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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