在exon 2的上游插入了loxP位点,而在exon 3下游则插入了loxP标记的诺卡因选择子 cassette。通过瞬时表达Cre重组酶,去除了诺卡因 cassette,使得exons 2和3被loxP位点包裹。靶向正确性通过Southern blot检测确认。(来源:J:132855)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count