在翻译起始点后,将lacZ插入子和floxed neo cassette插入到了第2外显子。通过在小肠和肝脏提取物中进行 Western Blot 分析,确认了没有蛋白质产物产生。(来源:J:132649)
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A lacZ cassette and floxed neo cassetted were inserted into exon 2 following the translation initiation site. The absence of protein product was confirmed by western blot analysis on intestine and liver extracts. (J:132649)