在翻译起始点后,将lacZ基因座和floxed neo基因座插入到了第2外显子。通过 Western blot 分析和免疫荧光检测,确认了没有蛋白质产物产生。(来源:J:132649)
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A lacZ cassette and floxed neo cassette were inserted into exon 2 following the translation initiation site. The absence of protein product was confirmed by western blot analysis and immunofluorescence. (J:132649)