在ES细胞中通过暂时的Cre表达,去除了exons 8、9和10。蛋白质产物的缺失通过在大脑、心脏、肾脏、肝脏、脾脏和睾丸的提取物中进行 Western blot 分析得到了确认(来源:J:132629)。
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Exons 8, 9 and 10 were removed by transient cre expression in ES cells. The absence of protein product was confirmed by western blot analysis on brain, heart, kidney, liver, spleen and testis extracts. (J:132629)