Fstl4^tm1Mno

A GFP-neo cassette with sequence encoding a 14 amino acid linker was inserted in frame into exon 2 replacing most of it. Only the first 4 amino acids of exon 2, the first coding exon, are expressed. The presence of the fusion transcript and protein was confirmed by RT-PCR and immunohistochemistry, respectively. The absence of the endogenous transcript was confirmed by RT-PCR. This is a null allele. (J:132129)