从2号到4号外显子被替换了新插入的cassette。通过在E13.5整个胚胎提取物中进行 Western blot 分析,确认了蛋白表达的缺失。(来源:J:132132)
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Exons 2 through 4 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on E13.5 whole embryo extracts. (J:132132)