在exon 2的编码区域被nls-lacZ/PGKneo cassette替换。通过RT-PCR和 Western blotting,我们确认了纯合小鼠中没有转录物和蛋白质的存在(来源:J:132020)
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The coding region in exon 2 was replaced with nls-lacZ/PGKneo cassette. The absence of transcripts and protein in homozygous mice was confirmed by RT-PCR and Western blotting. (J:132020)