通过 germ-line cre介导的重组来消除开放阅读框,通过在E14.5胚胎提取物中进行 Western blot 分析,确认了没有蛋白质产物的产生。(来源:J:131853)
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Germ-line cre-mediated recombination was used to remove the open reading frame. The absence of protein product was confirmed by western blot analysis on E14.5 embryo extracts. (J:131853)