从2号到4号外显子被替换成一个带有IRES-EGFP和neo cassette的序列。通过 Western blot 分析确认没有蛋白质产物产生。这是一项无蛋白产生基因的突变类型(来源:J:129608)。
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Exons 2 through 4 were replaced with an IRES-EGFP and neo cassette. The absence of protein product was confirmed by western blot analysis. This is a null allele. (J:129608)