A cassette containing a splice acceptor, frt-flanked neo cassette, floxed IRES-EGFP with a farnesyl modification, and human placental alkaline phosphatase was inserted into the ROSA locus. Expression of the membrane-bound EGFP occurs after flp-mediated excision and expression of the human placental alkaline phosphatase occurs after both flp- and cre-mediated recombination. (J:128980)