Tead1^tm1Hssk

A DNA fragment containing exon 2 and small segments of the flanking introns has been replaced by a loxP-flanked Pgk-neo-poly(A) cassette and a second cassette comprising an engrailed 2 splice acceptor (SA) followed by an internal ribosome entry site (IRES), the beta-galactosidase/neomycin fusion gene Betageo, and a polyadenylation signal. Reverse transcription- (RT-) PCR with primers for two downstream segments (exons 7-9 and 10-11) detects no transcripts in homozygous mutant embryos. (J:131426)