一段包含exon 2和邻近内含子小段的DNA片段已被替换成一个由loxP位点标记的Pgk-neo-poly(A)插件,以及第二个插件,包含engrailed 2的剪接接受体(SA)后面跟着的内质网进入信号(IRES),连接着betaglucuronidase/新霉素融合基因Betageo,以及poly(A)信号。用针对下游两个区域(exons 7-9和10-11)的反转录PCR(RT-PCR)在纯合突变胚胎中检测到没有转录本。(来源:J:131426)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
插入,基因内删除
隐性
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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