前两个外显子,包括起始的甲硫氨酸,被替换了新插入的neo cassette。通过脑组织的 Western blot 分析,证实了没有蛋白质产物产生。(来源:J:130903)
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The first two exons, including the start methionine, were replaced with a neo cassette. The absence of protein product was confirmed by western blot analysis on brain extracts. (J:130903)