Log In|Sign Up
中文
设计了一个靶向载体，包含猴类乙型肝炎生长因子（hbEGF）cDNA开放阅读框上游的loxP位点附带的STOP cassette。这个载体带有两个SV40polyA信号、一个frt标记的诺卡因抗性基因和一个转录终止子。通过C57BL/6来源的Bruce4 ES胚胎干细胞的电穿孔，这个构建被插入到Gt(ROSA)26Sor定位。广泛表达的DTR在上游的loxP位点STOP序列存在时被阻断。当与表达Cre重组酶的鼠杂交后，Cre存在的组织中STOP序列会被删除，从而允许DTR的表达。表达DTR的细胞在应用链霉索后会变得易受消除。来源：(J:131076)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
