Trim24^tm1.1Los

Exon 4 was floxed by introducing a loxP site upstream of the exon and inserting a loxP flanked PGK/TK neomycin cassette downstream of the exon. Chimeric mice were generated, crossed with a Cre-deleter strain and offspring were selected that had both exon 4 and the neo selection cassette removed. The deletion was also predicted to generate a frameshift mutation with a premature stop codon. Gene inactivation was confirmed by Western blot analysis of liver and testis protein. (J:130210)