在exon 4前面插入了一个loxP位点,并在exon后面插入了由loxP标记的PGK/TK Neo5383选择性载体。通过杂交产生了转基因小鼠,与Cre敲除纯合品系杂交,筛选出删除了Neo5383选择性载体但保留exon 4的后代。基因型通过基因组PCR进行了确认。(来源:J:130210)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129/Sv
Targeted
插入
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1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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