将含有Agouti、3'hprt和puro基因的磁带插入第8个内含子后,导致2到8号外显子的重复,通过肾和肠组织的 Western blot 分析确认了没有蛋白质产物产生。(来源:J:130191)
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A cassette containing the Agouti, 3'hprt and puro genes was inserted into intron 8 and resulted in a duplication of exons 2 through 8. The absence of protein product was confirmed by western blot analysis on kidney and intestine extracts. (J:130191)