Cfp^tm1.1Song

A targeting vector was constructed where a loxP site was inserted upstream of exon 3, and a FRT flanked neomycin resistance cassette followed by a loxP site was inserted downstream of exon 5. Upon recombination, the neomycin cassette inserted in the intended location but the upstream loxP site failed to recombine. FLPe recombination removed the FRT-flanked neomycin cassette located in intron 5 and endogenous gene expression was restored. (J:130108)