基因的编码外显子被β-半乳糖苷酶和抗氨苄青霉素基因替换。通过在纯合小鼠脾和骨髓中进行 Western blot 来确认基因失活。(来源:J:129871)
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The coding exons of the gene were replaced with beta-galactosidase and neomycin resistance genes. Gene inactivation was confirmed by western blot on spleen and bone marrow from homozygous mice. (J:129871)