Tead4^tm1Hssk

The gene was disrupted by insertion into exon 2, which encodes the DNA-binding TEA domain of the protein, of two functional cassettes: 1) a loxP-flanked Pgk-neo-poly(A) cassette, followed by 2) a cDNA encoding the beta-geo (E. coli beta-galactosidase/neomycin resistance) fusion protein flanked 5' by an internal ribosomal entry site (IRES) and 3' by a polyadenylation signal. Reverse transcription- (RT-) PCR using primers in exons 5 and 6 detects no transcripts from the mutant allele in homozygous E3.5 embryos. Whereas immunohistochemical analysis with antisera to TEAD4 stains all cell nuclei of 34-36-cell wild-type embryos, mutant embryos produce no signal. (J:130047)