Tg(Ins2-Chrm3*)1Jwe

The rat insulin 2 promoter was used to drive expression of the gene in beta cells. Chrm3 contains two modifications (an N-terminal 9 amino acid hemagglutinin epitope tag was added and a central portion of the third intracellular loop, Ala274 to Lys469, was deleted) that do not change its functionality but allow the researchers to distinguish it from the endogenous gene product. (J:129643)