通过Cre介导的重组,去除了18、19和20号外显子。这些外显子编码一个对于蛋白功能必不可少的SH2域。通过 Western blot 在肝脏组织中确认了蛋白产物的缺失。(来源:J:86400, J:128719)
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Cre-mediated recombination was used to delete exons 18, 19 and 20. These exons encode an SH2 domain essential for protein function. The absence of protein product was confirmed by western blot on liver tissue. (J:86400, J:128719)