Gt(ROSA)26Sor^{tm4(attB/attP)Sor}

A targeting construct with the SA-attB-PGKneo 4xpA-attP-lacZ-pA cassette, in which the STOP fragment is flanked by attB and attP sites (phi31 recognition sites), was inserted into the Gt(ROSA)26Sor. Expression of the lacZ gene is blocked by a STOP fragment (flanked by attB and attP sites) placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. PhiC31o-mediated recombination will excise the STOP and allow lacZ transcription. (J:128069)