部分exon 4(112bp)和exon 5被hrGFP和一个floxed neo cassette替换。通过在小鼠胚胎成纤维细胞中进行 Western Blot分析,确认了没有蛋白质产物产生。(来源:J:127492)
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Part of exon 4 (112 bp) and exon 5 were replaced with hrGFP and a floxed neo cassette. The absence of protein protduct was confirmed by western blot analysis on mouse embryonic fibroblasts. (J:127492)