在第13个内含子插入了一个LoxP位点,而在第14个内含子插入了带有PGK-neo cassette的区域。通过Cre介导的重组,第14个外显子被去除,产生了密码子框架移位的突变。外显子切除通过RT-PCR和从大肠提取的mRNA的测序分析得到了确认。 Western blot检测到了70 kDa的剪切片段。(来源:J:127452, J:156864)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
基因内删除
--
1
17
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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