在2号和3号外显子被替换成一个带有floxed neo cassette的元件,导致在BH3和跨膜区域之前的蛋白质部分缺失。通过 Western blot 分析确认了没有蛋白质产物产生。(来源:J:127533)
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Exons 2 and 3 were replaced with a floxed neo cassette leading to a protein truncation prior to the BH3 and transmemebrane domains. The absence of protein product was confirmed by western blot analysis. (J:127533)