将一个EGFP-IRES-creERT2的 cassette插入基因的第1外显子,同时伴随一个含loxP标记的诺卡因基因座。之后,通过与cre-deleter品系杂交,体内移除诺卡因基因。GFP表达由内源性启动子驱动,而cre表达则通过Tamoxifen处理激活,特别是在Lgr5表达的细胞中。(来源:J:127123)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
--
522

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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