Gt(ROSA)26Sortm2(Mirn150)Rsky mice were crossed to Tg(ACTFLPe)9205Dym mice to excise the frt-flanked IRES-EGFP cassette, producing mice slightly over-expressing Mirn150 (from the CAG promoter) in thymocytes. It is also possible to generate this allele from allele Gt(ROSA)26Sortm14.1(Mirn150)Rsky. (J:127234)