Del(2Hoxd4-Hoxd13)30Ddu

This allele involved the use of an embryonic stem cell line carrying an ectopic Hoxd11/lacZ transgene located between the resident Hoxd13 and Evx2 genes and flanked at the 3' end by a unique loxP site. A loxPneoloxP cassette was recombined on the same chromosome, downstream of resident Hoxd4. After treatment with Cre recombinase, the resultant deletion removed Hoxd4, Hoxd8, and Hoxd9, Hoxd10, Hoxd11, Hoxd12, and Hoxd13, but brought the ectopic Hoxd11/lacZ transgene back to the original Hoxd4 locus. Mice homozygous for this deficiency lacked Hoxd4-Hoxd13 loci. (J:92363)