通过Cre介导的重组,去除了包含编码219到376位氨基酸的cDNA和neo cassette的floxed片段,生成了一个带有终止密码子和polyadenylation位点的修改过的exon 7。这从蛋白质中移除了UIM区域。通过PCR基因分型来确认cDNA片段和neo cassette的去除(来源:J:126546)
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count