通过Cre介导的重组,去除了包含编码219到376位氨基酸的cDNA和neo cassette的floxed片段,生成了一个带有终止密码子和polyadenylation位点的修改过的exon 7。这从蛋白质中移除了UIM区域。通过PCR基因分型来确认cDNA片段和neo cassette的去除(来源:J:126546)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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