从exon 1的3'端到exon 2的5'端,这段区域被替换了新插入的neo cassette。通过脑部蛋白质提取物的 Western blot 分析,确实证实了没有蛋白质表达(来源:J:126869)
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The 3' half of exon 1 through the 5' half of exon 2 was replaced with a neo cassette. The lack of protein expression was confirmed by western blot analysis on protein extracted from the brain. (J:126869)