在第3和4外显子之间插入了一个由FRT引导的neo cassette,带有3'的loxP位点,然后在第6和7外显子之间插入了另一个loxP位点。通过使用Tg(ACTFLPe)9205Dym进行Flp介导的重组,以去除neo cassette。插入情况通过Southern blot分析和PCR基因分型来确认。(来源:J:126339)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入
--
1
--
8

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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